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Home arrow 3D Transfection

3D-Fect

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 3D Transfection: a new outlook for your cells!

3D-Fect™ is the newest reagent specifically developed to directly transfect cells cultured in 3D scaffold. 3D-Fect™ is suitable for all kind of scaffolds and cells.

3D matrices not only add a third dimension to cells’ environment, they also allow creating significant differences in cellular characteristics and behavior. Because 3D matrices are routinely used in basic research and therapeutic applications, we have developed 3D-Fect™ transfection reagent specific for scaffolds. In this way, scaffold-based 3D matrices combined with 3D-Fect™/DNA complexes, are colonized by cells to be transfected in a more natural environment. This method allows studying tissue engineering, tissue regeneration, tumor invasion, neural differentiation, cellular polarization, tissue formation, colonization, neurite growth…


3D-Fect™is the newest reagent specifically developed to directly transfect cells cultured in 3D scaffold.

This reagent is based on a novel technology that allows adding a third dimension to cell culture.

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  • Highly efficient: on cell lines and primary cells

  • Specific for 3D scaffolds

  • All type of nucleic acids: plasmid DNA, siRNA, shRNA...

  • Completely biodegradable

  • Serum compatible

  • Long term protein expression

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High transfection efficiency in various cells

  3D-Fect is highly efficient, even on primary cells and transgene expression is high and long.

Transfection of various cells on different scaffold

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 High secreted alkaline phosphatase expression
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Efficient with various scaffolds and matrices

 Name

 Type of Scaffold

 Collagen  Collagen-based scaffolds
 Collagen-derived  Collagen-derived scaffolds
 HA  Hyaluronic acid
 Millicel (PTFE)  Cell culture inserts (Millipore)
 PCL  Polycaprolactone
 PEG  Poly (Ethylene Glycol)
 PLGA  Poly (lactic-co-glycolic acid)
 PS  Poly (styrene)
 PU  Poly (urethane)

 

 

Outperforms competitors

 Comparison of RFP production in HEK-293 cells
 
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Comparison of secreted alkaline phosphatase production in COS-7 cells

 
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Product listing

TF200253D-Fect™ Sample Price:  Login
 
TF202503D-Fect™ 250µL Price:  Login
 65 transfections with 1µg of DNA
TF205003D-Fect™ 500µL Price:  Login
 

125 transfections with 1µg of DNA

TF210003D-Fect™ 1mL Price:  Login
 250 transfections with 1µg of DNA

 
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