Ab-DeliverIN^™

Antibody delivery reagent Ab-DeliverINTM is the first dedicated intracellular antibody delivery reagent. This lipid-based formulation is the first serum compatible reagent allowing the delivery of functional antibodies into living cells.

Due to its unique properties, Ab-DeliverINTM forms non-covalent complexes with antibodies through electrostatic and hydrophobic interactions. Consequently, chemical or genetic couplings are not necessary. In addition, delivered antibodies retain their structure and function. This feature makes Ab-DeliverINTM an exclusive reagent allowing the antibody to reach its intracellular target.

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Ab-DeliverIN^TM opens new fields of investigation in proteomics to elucidate complex molecular mechanisms or to design new potential therapies. For example, the intracellular delivery of blocking antibodies could inhibit and/or neutralize protein function.  

• Functionally active antibodies delivery

• Highly efficient in many primary cells and cell lines

• Serum compatible

• Biodegradable and no cytotoxicity

• Easy: straightforward protocol and ready-to-use

Intracellular localization of functional antibody

Ab-DeliverIN^TM permits the delivery of a functional antibody, making possible intracellular staining and protein localization studies.

Anti-giantin and anti-NPC antibodies localization

Highly efficient antibody transfection

Ab-DeliverIN^TM permits the delivery of antibodies in a large number of cells. It has been successfully tested on a large range of immortalized and primary cells including primary neurons and glial cells.

Delivery of antibodies in HEK cells and L929 fibroblasts

Delivery reagents comparison

In comparison with other protein delivery systems, Ab-DeliverIN^TM demonstrates higher delivery efficiency inside living cells.

Anti-NPC and non specific FITC-IgG delivery comparison

Serum compatible and fast delivery

Ab-deliverIN^TM is fully biodegradable and does not interfere with cellular mechanisms. In addition, it is compatible with serum containing culture media. You can obtain a significant amount of antibodies transported with no medium change which is less stressful for your cells. In vitro and in vivo experiments can be directly and quickly accomplished. Highest efficiencies can be achieved in lessthan 5 hours.

Delivery of proteins inside cells in presence of serum

Rapid and easy-to-use antibody transfection reagent

1. Mix Ab-DeliverIN^TM and antibodies
2. Incubate 10 min at room temperature
3. Add onto cells and incubate under standard conditions

Each kit contains 10 µg of FITC-labeled IgG at a concentration of 100 µg / mL in PBS.