B-Gal Assay Kit

ONPG and CPRG Beta-Galactosidase assay Lac Z is one of the most frequently reporter gene used in transfection experiments because of the gene product specific properties. Indeed, the Lac Z encoded protein, Beta-galactosidase (ß-gal), which hydrolyses galactosidase sugar such as lactose, is very stable, resistant to proteolytic degradation and easily tested.

All the necessary reagents are provided in these assay kits and offer rapid, simple and sensitive method to quantify spectrophotometrically the enzyme expression level in transfected cells.
OZ Biosciences has developed two optimized kits based on the use of ONPG or CPRG substrates.

• Convenient for all transfection assays

• Suitable for cultured cells and tissues

• Simple, rapid and easy-to-use

• Economical

• Optimized with stable buffer

What is the difference between ONPG and CPRG substrates?

ONPG ß-galactosidase assay kit:

This assay kit is ideal for quantitatively measure high expression level of ß-gal.
The levels of active ß-gal expression can be quickly measured by its catalytic hydrolysis of ONPG (o- nitrophenyl- ß-D-galactopyranoside), substrate to a bright yellow product.
(Absorbance at 405-420nm)

CPRG ß-galactosidase assay kit:

This assay kit is ideal for quantitatively measure low expression level of ß-gal.
The levels of active ß-gal expression can be quickly measured by its catalytic hydrolysis of CPRG (Chlorophenol red-ß-D-galactopyranoside), substrate to a dark red product. The high sensitivity of this substrate improves the measurement of ß-gal activity when the reporter gene expression is low.
(Absorbance at 570-595 nm)

ONPG and CPRG assay kit comparison