Bradford Protein Assay Kit

Bradford protein assay kit The Bradford Protein Assay Kit (B-Pak) is a straightforward and rapid procedure for determining the concentration of protein in solution. The B-Pak is based on the binding of Coomassie Brilliant Blue G-250 dye to the proteins and particularly basic and aromatic amino acids residues. The dye exists in three forms: cationic (red), neutral (green) and anionic (blue).

Under acidic conditions, the dye is predominantly in the protonated cationic form (red, Amax = 470 nm). When the dye binds to proteins, it is converted to a stable unprotonated form (blue, Amax = 595 nm). It is this blue unprotonated form that is detected at 595 nm to quantify the concentration of proteins.

• Accurate determination of protein concentration

• Ready to use prediluted standard protein

• Cheaper than competitors

• Convenient packaging

Fast and easy-to-use Bradford protein assay

This ready-to-use kit is provided with 1X reagent & prediluted standard which means that no dilution, no filtration or calculation are required. Simply process your assay in a few minutes.
B-Pak is provided with adapted protocols for all experimental conditions: micro and macroassays.

Protein concentration determination

Our kit allows an accurate determination of protein concentration, even in the presence of detergent (0.1%). Consequently, it is possible to determine total protein concentration in cell lysate or recombinant proteins stored in the presence of detergent. In addition this pak permits the determination of protein concentration ranging from 0.5 to 1500 µg/mL with a very high accuracy.

Protein concentration determination