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Calcium Phoshate Transfection Kit

calcium phosphate transfection kit

Ideal method for virus production

Calcium Phosphate Transfection Kit is perfect to transfect HEK 293 cells. This transfection method, first described by Graham and Van Der Ebb in 1973, has been optimized in order to reach higher transfection efficiency. The CaPO transfection kit is simple and easy to use. It allows reaching between 95 and 100% of HEK 293 transfected cells and a very high titer for virus production.

  • Ideal for virus production

  • High HEK 293 cells transfection efficiency

  • Suitable for producing recombinant proteins

  • Serum compatible

  • Simple, Ready-to-use and Rapid

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The ideal method for HEK 293 cells transfection

Calcium Phosphate Transfection Kit is optimized to be used for the transfection of HEK 293 cells with plasmid DNA. However, this procedure is also suitable for a variety of cell lines.

Transfection efficiency 

HEK 293 cells transfected with the Calcium Phosphate Transfection Kit
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Virus production

Virus production with the Calcium Phosphate Transfection Kit
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Protein production

Protein production with the Calcium Phosphate Transfection Kit
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Rapid and easy-to-use

1. Plate the cells in DMEM and incubate overnight
2. Change tissue culture medium 1-2h before transfection
3. Prepare the DNA solution in 1X HBS
4. Add the Calcium Chloride solution, mix and incubate 30 min
5. Add the complexes drop wise to your cells

 
Product listing
 
CP90000CaPO Transfection Kit Price:  Login
 100 transfections in 100 mm culture dish with 1 µg and 500 in 6-well plate with 4 µg

 
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