Calcium Phoshate Transfection Kit

Ideal method for virus production

Calcium Phosphate Transfection Kit is perfect to transfect HEK 293 cells. This transfection method, first described by Graham and Van Der Ebb in 1973, has been optimized in order to reach higher transfection efficiency. The CaPO transfection kit is simple and easy to use. It allows reaching between 95 and 100% of HEK 293 transfected cells and a very high titer for virus production.

Ideal for virus production
High HEK 293 cells transfection efficiency
Suitable for producing recombinant proteins
Serum compatible
Simple, Ready-to-use and Rapid

The ideal method for HEK 293 cells transfection

Calcium Phosphate Transfection Kit is optimized to be used for the transfection of HEK 293 cells with plasmid DNA. However, this procedure is also suitable for a variety of cell lines.

Transfection efficiency

HEK 293 cells transfected with the Calcium Phosphate Transfection Kit

Virus production

Virus production with the Calcium Phosphate Transfection Kit

Protein production

Protein production with the Calcium Phosphate Transfection Kit

Rapid and easy-to-use

1. Plate the cells in DMEM and incubate overnight
2. Change tissue culture medium 1-2h before transfection
3. Prepare the DNA solution in 1X HBS
4. Add the Calcium Chloride solution, mix and incubate 30 min
5. Add the complexes drop wise to your cells

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