DNA Markers

Plasmid DNA Markers

All OZ Biosciences markers are produced from certified high quality plasmid DNA employing nuclease-poor host bacteria. In addition the DNA fragments are further purified by de-proteination and desalting before being aliquoted and lyophilized. Their concentration is adjusted, so each vial contains 50µg of DNA.

List of DNA markers available

Size range of DNA markers

100bp DNA ladder (100 - 1000 bp)

Fragments: 1000 – 900 – 800 – 700 – 600 – 500 (2x) – 400 – 300 – 200 – 150 – 100 bp
Recommended for: Analysis of mid-sized DNA fragments from restriction analysis and plasmid vectors in the average size range
Features: 10 fragments with increments of 100bp with an additional 150bp fragment for the analysis of 100-200bp PCR-products. Two times concentrated 500bp-band for easy detection. Others bands are equimolar concentration (1:1 ratio). Needs 70-80mm distance in 1.2% - 1.5% agarose gels for complete separation of bands

Cat. Number: PF00100

100bp DNA ladder PLUS (100 - 5000 bp)

Fragments: 5000 – 4000 – 3000 – 2500 – 2000 – 1500 – 1000 – 900 – 800 – 700 – 600 – 500 (2x) – 400 – 300 – 200 – 150 – 100 bp
Recommended for: Analysis of mid- and large-sized DNA fragments from restriction analysis and plasmid vectors in the average size range
Features: In addition to the above mentioned 100bp-DNA ladder (60%), 40% of the total DNA-mass is an equimolar mixture of 6 high-molecular DNA fragments ranging from 1500 to 5000bp. On 1.0% - 1.5% agarose gels, the extended ladder looks like two separated, set-on-top DNA ladders.

Cat. Number: PF00200

1kbp DNA ladder (500 - 10000 bp)

Fragments: 10000 – 8000 – 6000 – 5000 – 4000 – 3000 – 2500 – 2000 – 1500 – 1000 – 500 bp
Recommended for: Analysis of larger DNA fragments and PCR-products.
Features: 11 fragments with increments of 2000, 1000 or 500bp. All bands are in equimolar concentration. Needs 70-800mm distance in 1.0% - 1.2% agarose gels for complete separation of bands.

Cat. Number: PF00300

ShortRun DNA Marker (500 - 2500 bp)

Fragments: 2500 – 2000 – 1500 (2x) – 1000 – 500 bp
Recommended for: Quick gel separation in high throughput applications.
Features: 5 fragments with 500bp increments. Needs 1.5-2.5mm distance in 1.2% - 1.5% agarose gels for complete separation of bands.

Cat. Number: PF00400

pBR328 Hinf / Bgl I (154 - 2176 bp)

Fragments: 2176 – 1766 – 1230 – 1033 – 653 – 517 – 453 – 394 – 298 – 234 – 220 – 154 bp
Recommended for: Quick gel separation in high throughput applications.Analysis of mid- and small-sized DNA fragments and PCR products
Features: Easily detectable double bands of 2176 / 1766bp and 1230 / 1033bp. Used on 1.2% - 1.5% agarose gels with fast spread-up of the fragments.

Cat. Number: PF00500

pUC18 Hpa II (26 - 501 bp)

Fragments: 501 – 489 – 404 – 353 – 242 – 190 – 147 – 110 – 89 – 67 – 34 – 26 bp
Recommended for: Analysis smaller PCR products.
Features: Used on 1.2% - 1.5% agarose gels with fast spread-up of the fragments.

Cat. Number: PF00700

pUC19 Msp I (26 - 501 bp)

Fragments: 501 – 489 – 404 – 331 – 242 – 190 – 147 – 111 – 110 – 67 – 34 – 26 bp
Recommended for: Analysis smaller PCR fragments.
Features: Contain a series of fragments in the low molecular range. Allows quick concentration by the easily detectable 501 / 489 and 111 / 110 double fragments. Needs only short separation distance on 1.5% - 2.2% agarose gels for the 501 to 110bp fragments.

Cat. Number: PF00700

pBR322 Hae III (8 - 587 bp)

Fragments: 587 – 540 – 502 – 458 – 434 – 267 – 234 – 213 – 192 – 184 – 124 – 123 – 104 – 89 – 80 – 64 – 57 – 51 – 21 – 18 – 11 – 8 bp
Recommended for: Gel analysis on 1.8% - 2.2% agarose gels.
Features: Large number of fragments in the low molecular range; mainly clustered in three areas of 3-4 fragments. Contains also very small fragments, which take longer for complete separation.

Cat. Number: PF00800

Lambda Hind III / phi-X 174 Hae III

Fragments: 23130 – 9416 – 6557 – 4361 – 2322 – 2027 – 1353 – 1078 – 872 – 603 – 564 – 310 – 281 – 271 – 234 – 194 – 125 – 118 – 72 bp
Recommended for: Gel analysis on 1.8% - 2.2% agarose gels.Wide range of fragment sizes.
Features: Fast spread-up of the fragments on 1.2% - 1.5% agarose gels

Cat. Number: PF00900

* All markers contain 1mL gel loading buffer (TAE, glycerol, bromophenol-xylenecyanol-blue) in order to dilute the 50 µg of DNA contained in each vial.