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Home arrow Products arrow DNA Transfection arrow Lipofection arrow DreamFect Stem

 

  DreamFect Stem

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Specific transfection reagent for Stem Cell

DreamFectTM Stem is an original, simple and efficient reagent especially developed to transfect multipotent stem cells with high efficiency and very low toxicity. It maintains their undifferentiated stage and their capacities to differentiate.

 

 

1.       High transfection efficiency for multipotent Stem Cells

2.       Minimized toxicity due to reagent biodegradability and low DNA amount required

3.       Does not affect phenotype and differentiation potential

4.       Serum Compatible

     5.       Simple, Ready-to-use and rapid (no specific buffer)

 

  •  DreamFect™ Stem exhibits high transfection efficiency

High transfection efficiency  

Several human stem cells  transfected with 0.5 μg of pVectOZ-GFP plasmid DNA and 1.5 μL per well of DreamFectTM Stem reagent in a 24-well plate. Percentage of GFP positive cells measured 48h post transfection by flow cytometry.

 

        
  • DreamFect™ Stem outperforms other transfection reagents

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Human mesenchymal stem cells were transfected with 0.5 μg of pVectOZ-GFP plasmid DNA and 1.5 μL of DreamFect™ Stem or with competitor’s reagent according to manufacturer’s manuals. Transfection efficiency was monitored by flow cytometry (Magnification x 40).

   

 

 

  • Rapid and easy-to-use transfection reagent

1/  Mix 3μL of DreamFectTM Stem Transfection reagent per μg of DNA (no specific dilution buffer needed)

2/ Incubate 20 min at room temperature

3/ Add the complexes to your cell growing in complete medium

      

 

Product listing
 
ST30500DreamFect Stem 500µl Price:  Login
 
ST31000DreamFect Stem 1ml Price:  Login
 
 
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