1/DreamFectTM Stem transfection efficiency on different stem cells
Adherent stem cells (MSC, AdSC, AFSC) were transfected with 0.5 μg of pVectOZ-GFP
plasmid DNA and 1,5 μL of DreamFect™
Stem per well in a 24-well plate. Transfection efficiency was assessed by
fluorescence microscopy 48h post transfection (Magnification x 200).
2/ DreamFectTM Stem minimizes toxicity
mesenchymal stem cells were transfected with 0.5 μg of pVectOZ-GFP plasmid DNA and 1.5 μL of DreamFectTM Stem or with
competitor's reagent according to manufacturer's manuals. 24 hours post-transfection, % of living
cells was analyzed by MTT assay.
3/DreamFectTM Stem does not affect the differenciation
Human mesenchymal stem
cells transfected with DreamFectTM Stem retain their potential to differentiate
into osteoblasts when cultured in osteogenic differentiation medium.
Two days after
transfection with DreamFect™ Stem, MSC were stimulated using an osteogenic
medium; and 18 days after stimulation, the cells were analyzed using alizarin
red staining. Bright-field microscopy images of the transfected nonstimulated
(A) and stimulated (B) MSCs 20 days after tr
ansfection (Magnification x 40).