purification and gene delivery in one integrated system
i-MICST™ Technology (Integrated Magnetic
Immuno-Cell Sorting and Transfection / Transduction) is a new platform that
allows to genetically modifying cells directly
on magnetic cell purification columns.
This technology combines cell isolation and
in one simple and reliable integrated system
and results in efficient and selective gene delivery to target cells.
i-MICST™ Technology allows you to reduce cell manipulation steps and save time and material.
Isolation and genetic modification in one reliable integrated system
Rapid, simple and ready to use
i-MICST™ Technology principle
How it works
i-MICST™ Technology requires:
cell separation systems (not provided by OZ Biosciences)
- Viro-MICST™ reagent for capturing virus
and infecting cells within the magnetic cell
This reagent is a new specific magnetic nanoparticles formulation issued from our MagnetofectionTM technology.
was developed in association with MACS® technology *
from Miltenyi Biotec.
demonstration were performed on MS and LS columns with MACS® separators
and the cell separation
reagents according to MACS® cell separation protocol.
Example protocol for transducing 106 cells on a MACS® MS column* with a MOI of 1.
Rapid, simple and ready-to-use
I / Cell preparation and Pre-enrichment of the target cells
Magnetically label your cells following the manufacturer's instructions
If cells to be purified < 50% of total cells, and/or cell purity<
90%, proceed to a
pre-enrichment step on non-modified column(s)
II/ Viral i-MICST™ procedure
Overview of the i-MICST™ Technology procedure
Please consult instruction manual for detailed informations.
Applications and Results
High level of target
cell transduction during purification
Significant improvement over the standard method