In vivo Transfection
|
In vivo Magnetofection™ has been designed for in vivo targeted transfection of various types of nucleic acids such as DNA, RNA, oligonucleotides.
OZ Biosciences offers two types of ready-to-use In vivo Magnetofection™ reagents:
-
In vivo PolyMag is a cationic polymer-based magnetic
nanoparticles formulation. It can be used with plasmid DNA, antisense
oligonucleotides, mRNA, shRNA and siRNA.
-
In vivo DogtorMag is a cationic lipid-based magnetic nanoparticles formulation. It associates Dogtor a specific cationic lipid and CombiMag magnetic nanoparticles.
This reagent is also suitable for plasmid DNA, antisense oligonucleotides, mRNA, shRNA and siRNA.
|
Ask for a trial kit
|
Benefits
-
Increased transfection efficiency
-
Magnetically targeted transfection to specific area
-
Universal - Suitable for all nucleic acids
-
Reduction of the systemic dissemination of vectors during injection
High efficiency for targeted delivery of DNA
In vivo Magnetofection™ has been used for local gene delivery in blood vessels. Plasmid DNA encoding luciferase complexed with PolyMag magnetic nanoparticles were injected into the ear veins of pig and ear artery of rabbit. A permanent magnet was placed respectively upstream and downstream the blood flow circulation and near the injection sites.
Results demonstrated that transgene was expressed at the magnet position indicating an efficient magnetic targeting.
In vivo PolyMag gene delivery
Transfection of ear artery endothelial cells
|
|
Fig1: High efficiency of In vivo PolyMag for targeted gene delivery after intravenous injection.
After 42h, reporter gene expression was found primarily at the magnet position site and to a lesser extent proximal and distal of the magnet. As control, the same vector composition was injected in the contralateral vessel without application of a magnet. No significant reporter gene expression was found at the topographically analogous positions.
From Plank et al., Expert Opin Biol Ther., 2003; 3:745-58 (1)
 More results for targeted delivery of DNA
|
High efficiency for DNA delivery in
hard-to-transfect tissue
Gene delivery and gene therapy in the guts are particular challenging due to the abundance of degradative enzymes, the presence of degraded nutriments and bacteria. In their study, Scherer and coll. successfully transfect a plasmid DNA encoding Lac-Z gene complexed with PolyMag magnetic nanoparticles into guts. Complexes were injected into the ileum lumen of rat and a permanent magnet was applied for 20 minutes.
|
Transfection in rat intestine
|
|
Complexes of DNA and PolyMag nanoparticles were applied to the ilea of rats in absence (A) or under the influence of a magnetic field (B). X-Gal staining was performed after 48h and revealed an efficient gene delivery only in the presence of magnet (B) at the macroscopic level (upper panel) and at the microscopic level (lower panel). In absence of magnetic field, only rare transfection events were observed (A). From Scherer et al., Gene Therapy., 2002; 9:102-109.
|
More results for cancer gene therapy, tissue targeting and gene silencing
| IV-KC30210 | In vivo PolyMag starting kit |
Price: Login | | |
Contains 500 µL of In vivo PolyMag + 1 Magnets set
|
| IV-PN30500 | In vivo PolyMag 500 µL |
Price: Login | | | 5-50 injections
|
| IV-PN31000 | In vivo PolyMag 1000 µL |
Price: Login | | | 10-100 injections
|
| IV-TK30210 | Trial kit In vivo PolyMag |
Price: Login | | | Contains 100 µL of In vivo PolyMag + 1 cylinder magnet (ø 10 mm)
|
| IV-MAG1 | Magnets set |
Price: Login | | | Contains 1 extra small cylinder (ø 2 mm), 1 small cylinder (ø 5 mm), 1 cylinder (ø 10 mm) and 1 square (18x18 mm) magnets
|
| IV-KC30220 | In vivo DogtorMag starting kit |
Price: Login | | |
Contains 500 µL of In vivo Dogtor + 500 µL of In vivo CombiMag + 1 Magnets set
|
| IV-DM30500 | In vivo DogtorMag 500 µL |
Price: Login | | |
Contains 500 µL of In vivo Dogtor + 500 µL of In vivo CombiMag
|
| IV-DM31000 | In vivo DogtorMag 1000 µL |
Price: Login | | |
Contains 1000 µL of In vivo Dogtor + 1000 µL of In vivo CombiMag
|
| IV-TK30220 | Trial kit In vivo DogtorMag |
Price: Login | | | Contains 100 µL of In vivo Dogtor + 100 µL of In vivo CombiMag + 1 cylinder magnet (ø 10 mm)
|
| IV-MAG1 | Magnets set |
Price: Login | | | Contains 1 extra small cylinder (ø 2 mm), 1 small cylinder (ø 5 mm), 1 cylinder (ø 10 mm) and 1 square (18x18 mm) magnets
|
|
|
Products 
Bibliographic References