A powerful  & unique transfection kit

This new kit is an association of DreamFect™ Gold and CombiMag reagents. DreamFect™ Gold formulation is a lipofection reagent achieving high transfection efficiency and high transgene expression level. CombiMag is a Magnetofection™ reagent: versatile and improving transfection efficiency without toxicity. The LipoMag Kit combines both of the advantages of lipofection and magnetofection transfection technique to transfect all kind of cells with the best efficiency!

BEAS-2B and COS-7 cells were transfected with Reagent L., DreamFect^TM Gold or with LipoMag Kit in a 24-well plate using 0.5µg of DNA. For LipoMag Kit: Dreamfect^TM Gold ratio and CombiMag ratio were fixed respectively at 3μL and 1μL per μg of DNA per well. Reagent L and DreamFect^TM Gold were used according to manufacturer instruction. GFP expression was assessed under fluorescent microscope 24H after transfection.

HeLa and COS 7 cells were first transfected with 0.25 µg of GFP expression plasmid mixed with LipoMag Kit (DreamFect™ Gold: 2 µL/µg DNA for HeLa and 3 µL/µg DNA for COS-7 – 1 µL CombiMag per µg DNA for both cell lines). 1 hour later, cells were sequentially transfected with RFP plasmid under the same conditions. Results were assessed by fluorescence microscopy 24 hours later.