This new kit is an association of DreamFect™ Gold and CombiMag reagents. DreamFect™ Gold formulation is a lipofection reagent achieving high transfection efficiency and high transgene expression level. CombiMag is a Magnetofection™ reagent: versatile and improving transfection efficiency without toxicity. The LipoMag Kit combines both of the advantages of lipofection and magnetofection transfection technique to transfect all kind of cells with the best efficiency!
Highest transfection efficiency
Achieve superior transgene expression than any other reagent
Universal: cell lines, primary cells and ideal for hard-to-transfect cells
For all nucleic acids applications
Non toxic & biodegradable
Simple, ready-to use and rapid
LipoMag Kit enhances number of transfected cells (GFP+)
Transfection efficiency A549 cells
LipoMag Kit increases protein production
Comparison with competitor
BEAS-2B and COS-7 cells were transfected with Reagent
L., DreamFectTM Gold or with LipoMag Kit in a 24-well plate using
0.5µg of DNA. For LipoMag Kit: DreamfectTM
Gold ratio and CombiMag ratio were fixed respectively at 3μL
and 1μL per μg of DNA per well.
Reagent L and DreamFectTM Gold were used according to manufacturer instruction. GFP
expression was assessed under fluorescent microscope 24H after transfection.
LipoMag Kit improves transfection efficiency in primary cells
Percentage of GFP+ cellscomparison with competitor
Co- and sequential transfection can be performed with LipoMag Kit
Sequential transfection in HeLa & COS-7
HeLa and COS 7 cells were first transfected with 0.25 µg of GFP
expression plasmid mixed with LipoMag Kit(DreamFect™ Gold: 2 µL/µg DNA for
HeLa and 3 µL/µg DNA for COS-7 – 1 µL CombiMag per µg DNA for both cell lines).
1 hour later, cells were sequentially transfected with RFP plasmid under the
same conditions. Results were assessed by fluorescence microscopy 24 hours