OZB Highlights
May 2013
Silencing and Rescue
siRNA delivery into BV2 murine microglia using PolyMag
Crehan et al. Blockade of CR1 prevents activation of rodent microglia.
Neurobiology of disease, 2013;54:139-49
Transfection of siRNA in Retinal Ganglion Cell with NeuroMag
D. Welsbie et al. Functional genomic screening identifies dual leucine zipper kinase as a key mediator of retinal ganglion cell death Proc Natl Acad Sci U S A, 2013;110(10):4045-50
Metabolic rescue of obese adipose derived stem cells through an original process of cytosol protein transfer using Pro-DeliverIN
Perez LM et al. Metabolic rescue of obese adipose-derived stem cells by Lin28/Let7 pathway
Diabetes, 2013 Feb 19 doi:10.2337/db12-1220
Previous Newsletters:
Transfection of primary mouse hippocampal neurons to study neurite outgrowth using MagnetofectamineTMTian et al. Lipid raft-dependent endocytosis of close homologue of adhesion molecule L1 (CHL1) promotes neuritogenesis.
J. Biol. Chem, 2012 ; 287(53):44447-63
Co-transfection of multiple plasmids or siRNA with MagnetofectamineTM in hard-to-transfect hepatoma cell line
Ito et al. Disruption of Stard10 gene alters the PPARa-mediated bile acid homeostasis.
Biochemica et Biophysica Acta, 2013 ; 1831(2):459-468
HEK293 transfection with MagnetofectamineTM to study the role of GLRB in heperekplexia
Chung et al. GLRB is third major gene of effect in hyperekplexia. Human Molecular Genetics, 2012, doi: 10.1093/hmg/dds498
Transfection of cortical neurons using NeuroMagC. Charrier et al. Inhibition of SRGAP2 function by its human-specific Paralogs induces neoteny during spine maturation.
Cell. 03 May 2012 ; Volume 149, Issue 4, 923-935
Delivery of antibodies into living neurons using Ab-DeliverinN.J. Douglas et al. Antibody Transfection into Neurons as a Tool to Study Disease Pathogenesis.
J. Vis. Exp. 2012 ; (67), DOI: 10.3791/4154
Interview with Cécile Charrier
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Transfection efficiency of MSC: A comparison of nucleofection and magnetofection
F Scheibe, et al. Nonviral gene delivery of erythropoietin by mesenchymal stromal cells.
Gene Therapy, 19(5):550-560, 2012.
Changes in cell behavior & transfection: A comparison of lipofection and nucleofection
Mello de Queiroz et al. Nucleofection induces non-specific changes in the metabolic activity
of transfected cells.
Mol. Biol. Rep (2012), 39, 2187-2194.
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Technical resources 
