X-Gal Staining Kit

Lac Z staining reagent

Lac Z is one of the most frequently reporter gene used in transfection experiments because of the gene product specific properties. Indeed, the Lac Z encoded protein, ß-galactosidase (ß-gal), which hydrolyses galactosidase sugar such as lactose, is very stable, resistant to proteolytic degradation and easily tested. This X-gal Staining Kit allows you to visualized histochemically ß-gal expression, though hydrolysis of X-gal (5-bromo-4-chloro-3-indoyl-ß-D-Galactopyranoside) which yields a blue precipitate.

All the necessary reagents are provided in this assay kit and offer a rapid, simple and sensitive method to determine the percentage of Lac Z transfected cells.

Consequently, cells transfected with ß-gal expressing plasmid appear blue following fixation and incubation with X-Gal substrate. Blue cells can be visualized by microscopy.